![]() Once your gel has finished running, a few preparatory steps are needed to ensure that your bands of DNA will transfer efficiently and accurately to the membrane. So even though Southern blotting is a longer experiment, it has many useful applications for many scientific disciplines! Along with this, Southern blotting can be used for sequencing DNA for both known and unknown sequences. For example, fragile X syndrome and sickle cell anemia are conditions that can be diagnosed using Southern blotting. Both RFLP and VNTR can be used for forensic analysis, paternity testing, and identifying and understanding mutations in the genome. Additionally, it is used when doing restriction fragment length polymorphism (RFLP) or variable number tandem repeat (VNTR) analyses. The reason we perform Southern blotting is to detect specific DNA molecules from other non-specific DNA molecules. Then the bands on the membrane can be visualized. Typically the membranes are made of nylon or nitrocellulose because these have the ability to transfer the DNA from the gel to the membrane using capillary action. Southern blotting takes the DNA from the gel slab after electrophoresis has finished running, and transfers it to a membrane. What is Southern blotting and why do we do it? ![]() Southern blotting was developed by Edwin Southern in the 1970’s at Edinburgh University, and has been used regularly in laboratories since to further analyze DNA bands separated during gel electrophoresis. ![]() Gel electrophoresis is a staple method in a lot of experiments, but to take your analysis one step further, you can look into trying out Southern blotting. ![]()
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